Edward G Dudley
Chen C., C. R. Lewis, K. Goswami, E. L. Roberts, C. Debroy, and E. G. Dudley. Identification and Characterization of Spontaneous Deletions within the Sp11-Sp12 Prophage Region of Escherichia coli O157:H7 Sakai. Appl. Environ. Microbiol. 2013 Jan 11. [Epub ahead of print].
Shariat, N., M. J. DiMarzio, S. Yin, L. Dettinger, C. H. Sandt, J. R. Lute, R. Barrangou, and E. G. Dudley. 2013. The combination of CRISPR-MVLST and PFGE provides increased discriminatory power for differentiating human clinical isolates of Salmonella enterica subsp. enterica serovar Enteritidis. Food Microbiol. 34:164-173.http://www.sciencedirect.com/science/article/pii/S0740002012002560
Weyrich L.S., O. Y. Rolin, S. J. Muse, J. Park, N. Spidale, M. J. Kennett, S. E. Hester, C Chen, E. G. Dudley, and E. T. Harvill 2012. A Type VI secretion system encoding locus is required for Bordetella bronchiseptica immunomodulation and persistence in vivo. PloS One 7:e45892
Verghese B., N. D. 3rd Schwalm, E. G. Dudley, and S. J. Knabel. 2012. A combined multi-virulence-locus sequence typing and Staphylococcal Cassette Chromosome mec typing scheme possesses enhanced discriminatory power for genotyping MRSA. Infect Genet Evol. 12:1816-1821.
D.M., E. G. Dudley, and R. F. Roberts. 2012. Technical note: development of a quantitative PCR method for monitoring strain dynamics during yogurt manufacture. J. Dairy Sci. 95:4868-4872.
Loquasto, J., R. Barrangou, E. G. Dudley, and R F. Roberts. 2011. The complete genome sequence of Bifidobacterium animalis subsp. animalis ATCC 25527 and comparative analysis of growth in milk with Bifidobacterium animalis subsp. lactis DSM 10140. J. Dairy Sci. 94:5864-5870.
Wen J., X. Deng, Z. Li, E. G. Dudley, R. C. Anantheswaran, S. J. Knabel, W. Zhang. 2011. Transcriptomic response of Listeria monocytogenes during the transition to the long-term-survival phase. Appl. Environ. Microbiol. 77:5966-5972.Liu, F., S. Kariyawasam, B. M. Jayarao, R. Barrangou, P. Gerner-Smidt, E. M. Ribot, S. J. Knabel, E. G. Dudley. 2011. Subtyping Salmonella serovar Enteritidis isolates from different sources using multilocus sequence typing based on virulence genes and CRISPRs. Appl. Environ. Microbiol. 77
Liu, F., R. Barrangou, E. Ribot, P. Gerner-Smidt, S. J. Knabel, and E. G. Dudley. 2011. Novel virulence gene and CRISPR multilocus sequence typing scheme for subtyping the major serovars of Salmonella enterica subspecies enterica. Appl. Environ. Microbiol. 77:1946-1956.
Hartzell, A., C. Chen, C. Lewis, K. Liu, S. Reynolds, E. G. Dudley. 2011. Escherichia coli O157:H7 of genotype LSPA 211111 and clade 8 are common clinical isolates within Pennsylvania. Foodborne Pathog. Dis.8:763-768.
Valadez, A., C. DebRoy, E. G. Dudley, and C. N. Cutter. 2011. Multiplex PCR detection of Shiga toxin-producing Escherichia coli strains belonging to serogroups O157, O103, O91, O113, O145, O111, and O26 experimentally inoculated in beef carcass swabs, beef trim, and ground beef. J. Food Prot. 74:228-239.
DebRoy, C., E. Roberts, A. Valadez, E. G. Dudley, and C. N. Cutter. 2011. Detection of Shiga toxin-producing Escherichia coli O26, O45, O103, O111, O113, O121, O145 and O157 serogroups by multiplex PCR of the wzx gene of the O-antigen gene cluster. Foodborne Pathog. Dis. 8:651-652.
Puri, A., and E. G. Dudley. 2010. Influence of indigenous eukaryotic microbial communities on the reduction of Escherichia coli O157:H7 in compost slurry. FEMS Microbiol. Lett. 313:148-154.
Chaudhuri, R.R., M. Sebaihia, J. L. Hobman, M. A. Webber, D. L. Leyton, M. D. Goldberg, A. F. Cunningham, A. Scott-Tucker, P. Ferguson, C. M. Thomas, G. Frankel, C. M. Tang, Dudley, E.G., I. S. Roberts, D. Rasko, M. J. Pallen, J. Parkhill, J. P. Nataro, N. Thomson, and I. R. Henderson. (2010). Complete genome sequence and comparative metabolic profiling of the prototypical enteroaggregative Escherichia coli strain 042. PLoS One 5(1):e8801.
Briczinski, E.P., J. R. Loquasto, R. Barrangou, E. G. Dudley, A. M. Roberts, R. F. Roberts. (2009). Strain-specific genotyping of Bifidobacterium animalis subsp. lactis by using single-nucleotide polymorphisms, insertions, and deletions. Appl. Environ. Microbiol. 75:7501-7508.
Liu, K., S. J. Knabel, and E. G. Dudley. (2009). Rearrangement hot spot (rhs) genes: potential markers in multilocus sequence typing for subtyping Escherichia coli O157:H7. Appl Environ Microbiol 75:5853-5862.
Barrangou, R., E. P. Briczinski, L. L. Traeger, J. R. Loquasto, M. Richards, P. Horvath, A. C. Coûté-Monvoisin, G. Leyer, S. Rendulic, J. L. Steele, J. R. Broadbent, T. Oberg, E. G. Dudley, S. Schuster, D. A. Romero, R. F. Roberts. (2009). Comparison of the complete genome sequences of Bifidobacterium animalis subsp. lactis DSM 10140 and Bl-04. J. Bacteriol. 191:4144-4151.
Dudley, E. G. (2008). In vivo expression technology and signature-tagged mutagenesis screens for identifying mechanisms of survival of zoonotic foodborne pathogens. Foodborne Path. Dis. 5:473-85.
De Luna, M. G., A. Scott-Tuckler, M. Desvaux, P. Ferguson, N. P. Morin, E. G. Dudley, S. Turner, J. P. Nataro, P. Owen, and I. R. Henderson. (2008). The Escherichia coli biofilm-promoting protein Antigen 43 does not contribute to intestinal colonization. FEMS Microbiol. Lett. 284: 237-246.
My program uses molecular biology, biochemistry, and molecular biology techniques to better understand the biology and evolution of foodborne pathogens, and to develop improved methods of tracking the spread of these organisms from farm-to-fork.
We focus primarily on Escherichia coli O157:H7 and Salmonella enterica. For E. coli, we have been interested in developing effective molecular subtyping methods for both epidemiologic investigations and for tracing the evolution of this organism. We recently reported on a cluster of genes, collectively referred to as Recombination Hot Spot (rhs) genes, which permitted the separation of certain O157:H7 strains by multilocus sequence typing (MLST) (Lui et al., 2009). We were funded in 2010 by the USDA-NIFA to sequence a large number of E. coli O157:H7 genomes with the goal of identifying additional sequence variations in a larger collection. We are also interested in the role bacteriophage, or bacteria viruses, have played in the evolution of E. coli O157:H7. Many virulence genes, including that for Shiga toxin, were acquired by bacteriophage transduction however these phage may also mediate the regulation of virulence genes as well.
Our work with Salmonella enterica is primarily directed at designing improved molecular subtyping methods for tracking outbreaks, in collaboration with Dr. Stephen Knabel’s laboratory (Penn State). We have been particularly interested in CRISPRs, or Clustered Regularly Interspaced Short Palindromic Repeats, which are elements shown in other organisms to mediate defense against foreign DNA including bacteriophage and plasmids. Because of their rapid evolution, we are investigating the utility of CRISPRs in MLST schemes for differentiating strains from the most common serovars of S. enterica. We are also interested in studying the mechanisms of CRISPR function and evolution in S. enterica.